9/1/2023 0 Comments Chromosome scaffold proteinHowever, our knowledge of the precise relationship between composition of NuMat and MiCS remains limited. This arrangement of DNA loops in mitotic chromosomes is similar to the DNA loops that are attached to NuMat in interphase nucleus ( 14, 15).Ĭonsidering that NuMat and mitotic chromosome scaffold (MiCS) are comparable biochemical structures, it is reasonable to expect that at least certain components of the NuMat are retained in MiCS ( 16, 17). Early evidence for transmission of information for higher order chromatin organization through mitosis came from the pioneering work by Laemmli and co-workers, who showed that in histone depleted mitotic chromosomes, DNA loops are attached to a proteinaceous scaffold. For example, chromosome positions are transmitted through mitosis and non-random higher order chromatin arrangements can be stably propagated over many cell cycles ( 12, 13). Several studies suggest that along with this, the information for 3D organization of chromatin in the nucleus is also stably inherited in daughter cells. One of the mechanisms by which this is accomplished is by bookmarking of genes mediated by locus specific retention of transcription factors and deposition of heritable epigenetic marks ( 9 – 11). Most genes are thought to be silenced during cell division but are precisely re-activated in daughter cells to achieve gene expression pattern that is indistinguishable from the mother cell ( 7, 8). However, during open mitosis the three-dimensional organization of the nucleus breaks down as the chromosomes condense to enable faithful segregation of duplicated chromatids into daughter cells. NuMat, along with facilitating the packaging of DNA, organizes the nuclear microenvironment in a way that transcription, replication, splicing and repair are executed in a coordinated manner over the highly compacted DNA. Specific attachment of chromatin fibers to NuMat create a higher order structure made up of discrete topological loop domains ( 5, 6). During interphase, interior of the nucleus is organized on a ribonucleo-protein network known as nuclear matrix (NuMat) 1 ( 4). Genetic information that is encoded in a linear DNA sequence, needs to be organized in three-dimensional space of the nucleus in the form of chromatin for appropriate expression of genes ( 1 – 3). Our study clearly indicates that the features of nuclear architecture, in the structural context of NuMat, are retained in MiCS and possibly play an important role in maintenance of cell lineage specific transcriptional status during cell division and thereby, serve as components of cellular memory. Although several transcription factors involved in functioning of interphase nucleus are present exclusively in NuMat, protein components responsible for assembly of membrane-less nuclear bodies are uniquely retained in MiCS. Chromatin/RNA binding proteins with hydrolase and helicase activity are highly enriched in NuMat as well as MiCS. ![]() Proteins of the NuMat/MiCS have large dynamic range of MS signal and were detected in sub-femtomolar amounts. ![]() Our study elucidates that as much as 67% of the NuMat proteins are retained in the MiCS indicating that the features of nuclear architecture in interphase nucleus are retained on the mitotic chromosomes. ![]() We compared the proteome of nuclease and high salt resistant fraction of interphase nucleus known as nuclear matrix (NuMat) and an equivalent biochemical fraction in the mitotic chromosome known as mitotic chromosome scaffold (MiCS). This necessitates that the features of nuclear architecture and DNA topology persist through mitosis. Chromatin condenses several folds to form mitotic chromosomes during cell division and decondenses post-mitotically to reoccupy their nuclear territory and regain their specific transcriptional profile in a precisely lineage specific manner.
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9/1/2023 0 Comments J.cole no role models download![]() That leave a toothbrush at your crib loveĪnd you ain't gotta wonder whether that's your kid love ![]() I want a real love, dark skinned Aunt Viv love I got defensive and said, "Nah, I was the same without it."īut then I thought back, back to a better meīefore I was a B-list celebrityīefore I started callin' bitches "bitches" so heavilyīack when you could get a platinum plaque without no melody She think I'm spoiled and I'm rich 'cause I can have any bitch I came fast like 9-1-1 in white neighborhoods Then hang the phone up and proceed to lay the wood "Sorry I'm never comin' home, I'ma stay for good." This bitch'll make you call your girl upĪnd tell her, "Hey, what's good?" Last night I was gettin' my feet rubbedīy the baddest bitch, not Trina but I swear to God Searchin' through my memory, my memory, I couldn't find one Lame niggas can't tell the differenceĭon't save her, she don't wanna be saved Martin Luther King woulda been on Dreamville Listen, even back when we was broke, my team ill Prophecies that I made way back in the Ville, fulfilled I get my bitch pregnant, I'ma be a better you Lily D.First things first: rest in peace Uncle Philįor real, you the only father that I ever knew.Vela on Analysis on Money Trees by Kendrick Lamar Juliana Maria Buchser on Walking Through The Woods of Mac Miller’s Mindįairy on “My Heart is Buried in Venice…” ok Ricky, I’m on my way to get it julianacalifornia on Walking Through The Woods of Mac Miller’s Mind.This shows that attractiveness can take a women very far in the lifestyle that he lives, because famous men like him prioritize it so much. This experience shows how he plays into the role that allows morally poor women to thrive by choosing a girl that he finds extremely attractive over a girl that has a good personality. Sorry I’m never comin’ home, I’ma stay for good'”. Not Trina but I swear to God, this b****’ll make you call your girl up “Last night I was gettin’ my feet rubbed by the baddest b**** In another sexual encounter with a women, Cole shows how he feels “these type” of women should be treated, but then shows how men feed into this role and make it possible. It is clear he believes they are only talking to him because he is famous, and want to attach themselves to him, which he symbolizes him preventing by taking his shirt back. In this excerpt he dehumanizes the women he had a sexual experience by calling them birds in order to enforce that idea that the majority of women in Hollywood do not deserve the type of respect you would give to an ordinary person. Takin’ off her skirt, let her wear my shirt, ‘fore she leave You think if I didn’t rap she would flirt back? She deserved that, she a bird, it’s a bird trap “Out in Hollywood bringin’ back five or six hoesį*** ’em then we kick ’em to the do’, n****, you know how it go Cole tells a story about an encounter he has with a woman in Hollywood that he believes to be morally poor. The repetition shows how he believes the majority of woman do not deserve to be “saved” in Hollywood. “Don’t save her, she don’t wanna be saved.ĭon’t save her, she don’t wanna be saved.ĭon’t save her, she don’t wanna be saved.” The main chorus of this song demonstrates this perfectly, While the theme and the way he portrays it is definitely condescending towards women, he also talks about how men, including himself, play into this role and make it possible for women who he morally attacks to be prosperous. He develops a theme that some women are good individuals and respectable, but that most aren’t, and that is takes a smart man to decipher and decide between the two. Cole develops another theme by attacking the people in Hollywood, specifically woman. He shows his belief on how important it is to have a male parental figure when he says “I get my b**** pregnant I’ma be a better you.” His desire to be an outstanding Dad for his child displays his belief in importance of a strong Dad in a boy’s life. The line “you the only father that I ever knew” implies that he did not have a male parental figure growing up, and the fact that he relied on a TV show to gain a positive male influence shows the lack of a male parental figure in his life, and how important it is to have one. Cole immediately addressing Uncle Phil shows the importance to him of a male figure who stepped up as a parental figure. This is an allusion to a TV show called “Fresh Prince of Bellaire”, in which a character named Uncle Phil looks after a young boy for the large duration of his childhood due to him not having a father figure. ![]() “First things first: rest in peace Uncle Phil ![]() Cole immediately addresses this by saying, The first theme that I am going to focus on is the one of lack of male role models. Cole portrays multiple themes is this song, and I am going to focus on two of them. Cole, featured on his album 2014 Forest Hills Drive. |
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